Cloned (Comment) | Organism |
---|---|
expressed in Aspergillus sojae | Mucor circinelloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28.3 | - |
D-glucose | recombinant enzyme, apparent value, at pH 7.0 and 37°C | Mucor circinelloides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
- |
Mucor circinelloides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mucor circinelloides | - |
- |
- |
Mucor circinelloides NISL0103 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Mucor circinelloides |
Purification (Comment) | Organism |
---|---|
Toyopearl-butyl 650C column chromatography and SP-Sepharose column chromatography | Mucor circinelloides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Mucor circinelloides | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose + 2,6-dichlorophenolindophenol | 0.44% activity compared to D-glucose | Mucor circinelloides | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-galactose + 2,6-dichlorophenolindophenol | 0.44% activity compared to D-glucose | Mucor circinelloides NISL0103 | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | the enzyme has high specificity toward D-glucose | Mucor circinelloides | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-glucose + 2,6-dichlorophenolindophenol | the enzyme has high specificity toward D-glucose | Mucor circinelloides NISL0103 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + 2,6-dichlorophenolindophenol | 1.53% activity compared to D-glucose | Mucor circinelloides | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
D-xylose + 2,6-dichlorophenolindophenol | 1.53% activity compared to D-glucose | Mucor circinelloides NISL0103 | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + 2,6-dichlorophenol indophenol | 1.09% activity compared to D-glucose | Mucor circinelloides | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltose + 2,6-dichlorophenol indophenol | 1.09% activity compared to D-glucose | Mucor circinelloides NISL0103 | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltotetraose + 2,6-dichlorophenolindophenol | 0.66% activity compared to D-glucose | Mucor circinelloides | maltotetraono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
maltotriose + 2,6-dichlorophenolindophenol | 0.88% activity compared to D-glucose | Mucor circinelloides | maltotriono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
mannose + 2,6-dichlorophenolindophenol | 0.66% activity compared to D-glucose | Mucor circinelloides | mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
additional information | no activity with sucrose | Mucor circinelloides | ? | - |
? | |
additional information | no activity with sucrose | Mucor circinelloides NISL0103 | ? | - |
? | |
trehalose + 2,6-dichlorophenolindophenol | 0.22% activity compared to D-glucose | Mucor circinelloides | ? + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 69537, calculated from amino acid sequence | Mucor circinelloides |
? | x * 90000-130000, SDS-PAGE | Mucor circinelloides |
Synonyms | Comment | Organism |
---|---|---|
FAD-dependent GDH | - |
Mucor circinelloides |
FAD-GDH | - |
Mucor circinelloides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Mucor circinelloides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | about 70% activity at 25°C, about 90% activity at 30°C, about 95% activity at 35°C, 100% activity at 40°C, about 65% activity at 45°C, less than 20% activity at 50°C | Mucor circinelloides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | the enzyme remains stable after 15 min at 25-40°C, then activity drops to about 70% at 50°C and is completely lost at 55°C | Mucor circinelloides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
749.7 | - |
D-glucose | recombinant enzyme, apparent value, at pH 7.0 and 37°C | Mucor circinelloides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | - |
Mucor circinelloides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
approximately 80% of the enzymatic activity is retained after incubation at 40°C for 15 min at pH 5.0. Treatment at 25°C for 16 h shows that the enzyme is stable from pH 3.5 to 7.0 | Mucor circinelloides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Mucor circinelloides |